Difference between revisions of "Part:BBa K611010"

Latest revision as of 01:23, 25 September 2011

LacI Repressor Characterization Construct

This part is used to characterize mutants of BBa_C0012 (LacI). A mutant of BBa_C0012 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI and PstI. One can then measure several of the mutant's properties through its regulation of GFP expression. Concentration of the added LacI mutant can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1216
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1156
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 870

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 <partinfo>BBa_K611010 short</partinfo>
-This part is used to characterize mutants of BBa_C0012 (LacI). A mutant of BBa_C0012 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI PstI. One can then measure several of the mutant's properties through its regulation of GFP expression.  Concentration of the added LacI mutant can be varied through different arabinose concentrations as long as AraC is present.
+This part is used to characterize mutants of BBa_C0012 (LacI). A mutant of BBa_C0012 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI and PstI. One can then measure several of the mutant's properties through its regulation of GFP expression.  Concentration of the added LacI mutant can be varied through different arabinose concentrations as long as AraC is present.
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