Difference between revisions of "Part:BBa K649301"
| Line 5: | Line 5: | ||
Ptrc and RBS are from pTrc99A.<br /> | Ptrc and RBS are from pTrc99A.<br /> | ||
| − | + | You should NOT ligate this part into high-copy vector.<br /> | |
| + | |||
| + | In our test, ligated into pSB1C3, <span style="font-style:italic;">E.coli</span> dosen't glow after 24 hours incubation.<br /> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 06:13, 29 September 2011
Ptrc-RBS-rocF
rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.
You should NOT ligate this part into high-copy vector.
In our test, ligated into pSB1C3, E.coli dosen't glow after 24 hours incubation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]