Difference between revisions of "Part:BBa K530031"
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<partinfo>BBa_K530031 short</partinfo> | <partinfo>BBa_K530031 short</partinfo> | ||
− | Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and URA3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. | + | Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and URA3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector. |
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Latest revision as of 01:42, 25 September 2011
pRSBB406 URA3 Integrating Yeast Shuttle Vector
Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This is chromosomal integrating vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1676
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1085
Illegal BsaI.rc site found at 3356
Illegal SapI site found at 2273
Illegal SapI.rc site found at 932