Difference between revisions of "Part:BBa K530036"

Latest revision as of 01:04, 25 September 2011

pRSBB423 HIS3 2Micron Episomal Yeast Shuttle Vector

Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 5258
Illegal PstI site found at 1183
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1183
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 899
Illegal BglII site found at 959
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 5258
Illegal PstI site found at 1183
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 5258
Illegal PstI site found at 1183
Illegal NgoMIV site found at 1748
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 3428
Illegal SapI site found at 2345

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 __NOTOC__
 <partinfo>BBa_K530036 short</partinfo>
-HIS3
+Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and HIS3 selectable marker for ''S. cerevisiae''.  Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 16: @@
 <partinfo>BBa_K530036 parameters</partinfo>
 <!-- -->
+===Plasmid Map===
+[[Image:pRS423.png]]