Difference between revisions of "Part:BBa K530035"

 
 
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Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and URA3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
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===Plasmid Map===
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[[Image:pRS416.png]]

Latest revision as of 00:57, 25 September 2011

pRSBB416 URA3 CEN/ARS Yeast Shuttle Vector Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1085
    Illegal BsaI.rc site found at 3356
    Illegal SapI site found at 2273
    Illegal SapI site found at 4377
    Illegal SapI.rc site found at 932


Plasmid Map

PRS416.png