Difference between revisions of "Part:BBa K530034:Experience"
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===Applications of BBa_K530034=== | ===Applications of BBa_K530034=== | ||
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| + | ==Characterization by iGEM uOttawa 2017== | ||
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| + | iGEM uOttawa 2017 explored if regulated recruitment of dcas9 Mxi1 using synthetic guide RNA is a viable alternative to conventional transcriptional regulatory modules in genetic network engineering. | ||
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| + | The main model organism used by our lab and many others is S. Cerevisiae. The absence of available yeast backbones from the Registry and the relatively few available in the Distribution Kit is a significant problem that we sought to investigate. This shuttle vector, BBa_K530034 addresses the issue by enabling the replication and propagation of genetic material in both e coli and yeast. | ||
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| + | The existence of this part presented a theoretical solution to bridging the gap between e coli biobricks and the limited compatibility in yeast. However, there is a lack of information regarding its practical usage and application. Therefore, iGEM uOttawa 2017 aimed to improve the characterization of Leu2 CEN/ARS yeast shuttle vector by examining its functionality and applicability to both e coli and yeast systems as a part of our project. | ||
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| + | A central part of our project, dcas9 Mxi1, was examined in both e coli and yeast. Our plasmid incorporated the use of Leu2 CEN/ARS yeast shuttle vector that also contained an ampicillin selection (see in plasmid design). | ||
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| + | [[File:UOttawa dcas9 plasmid design.jpg|thumb|800px|center]] | ||
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| + | We have proven that this yeast shuttle vector is compatible with ecoli strain DH5alpha as well as yeast strain W303 as shown by the growth on the ampicillin selection plate (ecoli right) and leucine selection plate (yeast left). | ||
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| + | [[File:UOttawa amp and leu plates.jpeg|thumb|800px|center]] | ||
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| + | In conclusion, our improved informational characterization provides practical support for the use of yeast shuttle vectors as a solution to the long existing compatibility issue. | ||
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| + | ==Contribution Markup== | ||
| + | Group: uOttawa, 2017 | ||
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| + | Author: Sakib Kazi and Yuchen Luo | ||
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| + | Summary: We incorporated a plasmid combining Leu2 CEN/ARS yeast shuttle vector and a dcas9 Mxi1 part into the e coli strain DH5alpha and yeast strain w303 and proved its functionality. The success of the experiment provides information regarding practical application of this useful part. | ||
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| + | Uploads: For further information regarding the project, please visit http://2017.igem.org/Team:uOttawa | ||
| + | Please see images of growth above. | ||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:56, 26 October 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K530034
Characterization by iGEM uOttawa 2017
iGEM uOttawa 2017 explored if regulated recruitment of dcas9 Mxi1 using synthetic guide RNA is a viable alternative to conventional transcriptional regulatory modules in genetic network engineering.
The main model organism used by our lab and many others is S. Cerevisiae. The absence of available yeast backbones from the Registry and the relatively few available in the Distribution Kit is a significant problem that we sought to investigate. This shuttle vector, BBa_K530034 addresses the issue by enabling the replication and propagation of genetic material in both e coli and yeast.
The existence of this part presented a theoretical solution to bridging the gap between e coli biobricks and the limited compatibility in yeast. However, there is a lack of information regarding its practical usage and application. Therefore, iGEM uOttawa 2017 aimed to improve the characterization of Leu2 CEN/ARS yeast shuttle vector by examining its functionality and applicability to both e coli and yeast systems as a part of our project.
A central part of our project, dcas9 Mxi1, was examined in both e coli and yeast. Our plasmid incorporated the use of Leu2 CEN/ARS yeast shuttle vector that also contained an ampicillin selection (see in plasmid design).
We have proven that this yeast shuttle vector is compatible with ecoli strain DH5alpha as well as yeast strain W303 as shown by the growth on the ampicillin selection plate (ecoli right) and leucine selection plate (yeast left).
In conclusion, our improved informational characterization provides practical support for the use of yeast shuttle vectors as a solution to the long existing compatibility issue.
Contribution Markup
Group: uOttawa, 2017
Author: Sakib Kazi and Yuchen Luo
Summary: We incorporated a plasmid combining Leu2 CEN/ARS yeast shuttle vector and a dcas9 Mxi1 part into the e coli strain DH5alpha and yeast strain w303 and proved its functionality. The success of the experiment provides information regarding practical application of this useful part.
Uploads: For further information regarding the project, please visit http://2017.igem.org/Team:uOttawa Please see images of growth above.
User Reviews
UNIQ29c9033f88249cd3-partinfo-00000000-QINU UNIQ29c9033f88249cd3-partinfo-00000001-QINU