Difference between revisions of "Part:BBa K530032"
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− | HIS3 | + | Yeast shuttle vector for propagation in ''E. coli'' and transformation into ''S. cerevisiae''. Contains an Ampicillin resistance marker for ''E. coli'' and HIS3 selectable marker for ''S. cerevisiae''. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector. |
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+ | ===Plasmid Map=== | ||
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+ | [[Image:pRS413.png]] |
Latest revision as of 00:40, 25 September 2011
pRSBB413 HIS3 CEN/ARS Yeast Shuttle Vector
Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1008
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 899
Illegal BglII site found at 959 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1748
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3428
Illegal SapI site found at 2345
Illegal SapI site found at 4449