Difference between revisions of "Part:BBa K590051"

Revision as of 21:27, 24 September 2011

Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) Fusion

This part encodes a direct gene fusion product of the two enzymes, Acyl-ACP Reductase(AAR) and Aldehyde Decarbonylase (ADC), in a linear configuration with a glycine-serine linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. For more information, refer to [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team wiki]

Usage and Biology

2011 UW iGEM Team has constructed this fusion product to optimize the alkane production developed by the team by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production

Direct fusion of two proteins using a glycine-serine linker.

GC-MS data shows that this system wasn't successful at optimizing the alkane production. For more info, refer to 2011 UW iGEM Team wiki

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590051 short</partinfo>
-This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase(AAR)] and  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)], in a linear configuration with a glycine-serine linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
+This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase(AAR)] and  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)], in a linear configuration with a glycine-serine linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. For more information, refer to [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team wiki]
 ===Usage and Biology===
-We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together.
+UW iGEM Team has constructed this fusion product to optimize the alkane production developed by the team by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production
 [[Image:Washington2011_Direct_Fusion_Localization_Image.png|left|430px|thumb|Direct fusion of two proteins using a glycine-serine linker.]]
-[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|430px|thumb|Contrary to what was expected, our GCMS data demonstrated that direct fusion has a negative effect on alkane production.]]
+[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|430px|thumb|GC-MS data shows that this system wasn't successful at optimizing the alkane production. For more info, refer to 2011 UW iGEM Team wiki]]