Difference between revisions of "Part:BBa K590051"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together. | ||
[[Image:Washington2011_Direct_Fusion_Localization_Image.png|left|430px|thumb|Direct fusion of two proteins using a glycine-serine linker.]] | [[Image:Washington2011_Direct_Fusion_Localization_Image.png|left|430px|thumb|Direct fusion of two proteins using a glycine-serine linker.]] | ||
[[Image:Washington2011_Alkane_Production_Localization_Graph1.png |right|430px|thumb|Contrary to what was expected, our GCMS data demonstrated that direct fusion has a negative effect on alkane production.]] | [[Image:Washington2011_Alkane_Production_Localization_Graph1.png |right|430px|thumb|Contrary to what was expected, our GCMS data demonstrated that direct fusion has a negative effect on alkane production.]] | ||
Revision as of 21:22, 24 September 2011
Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) Fusion
This part encodes a direct gene fusion product of the two enzymes, Acyl-ACP Reductase(AAR) and Aldehyde Decarbonylase (ADC), in a linear configuration with a glycine-serine linker for co-localization optimization of ADC alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
Usage and Biology
We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]