Difference between revisions of "Part:BBa K542007"

Revision as of 18:52, 28 September 2011

Unregulated TetR with pTet Regulated Arg-tagged ECFP

Made by assembling BBa_P0440 with BBa_K331033.

The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter (BBa_R0010) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.

Intermediate part to assemble the "Co-localization Testing Construct".

Datasheet for the test construct Part BBa_K542008 in E. coli strain DH5alpha demonstrating this is a functional intermediate in that construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 Made by assembling [https://parts.igem.org/Part:BBa_P0440 BBa_P0440] with [https://parts.igem.org/Part:BBa_K331033 BBa_K331033].
+<br><br>
 The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.
+<br><br>
 Intermediate part to assemble the "Co-localization Testing Construct".
+<br><br>
+Datasheet for the test construct <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct.
+<br><br>
+[[image:uoflECdatasheet.png|150px]]
 <!-- Add more about the biology of this part here