Difference between revisions of "Part:BBa K649300"
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Original rocF has two EcoRI sites in ORF, so we changed these sites.
 
Original rocF has two EcoRI sites in ORF, so we changed these sites.
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To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click here.
  
  

Revision as of 21:07, 5 October 2011

Arginase (rocF)

This part has only rocF obtained from B.subtilis 168 by colony-PCR.
rocF is the cording region of Arginase(EC:3.5.3.1), catalyzing the reaction from arginine and water to urea and ornithine.

Original rocF has two EcoRI sites in ORF, so we changed these sites.

To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click here.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]