Difference between revisions of "Part:BBa K542004"

Revision as of 19:26, 23 September 2011

pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD

Made by assembling BBa_K542001 with BBa_K542003.

Note that the pLacI promoter (BBa_R0010) is regulating the Lumazine Synthase gene, not the pBAD promoter (BBa_I13453) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible.
The microcompartment portion to the "Co-localization Construct".

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 961
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 901
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K542004 short</partinfo>
@@ Line 5: / Line 4: @@
 Made by assembling [https://parts.igem.org/wiki/index.php?title=Part:BBa_K542001 BBa_K542001] with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K542003 BBa_K542003].
-Note that the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible.
+Note that the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. <br>
 The microcompartment portion to the "Co-localization Construct".