Difference between revisions of "Part:BBa K516999"

Revision as of 15:32, 23 September 2011

pSB1C3 with RBS-RFP-TT between SpeI-PstI

When the SpeI-PstI DNA fragment from BBa_J61002, containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into pSB1C3 (digested SpeI-PstI) the resulting plasmid is BBa_K516999. See pSB1C3 for detailed feature information.

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Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 885
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2918
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal NotI site found at 878
Illegal NotI site found at 2924
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2918
Illegal XhoI site found at 1902
Illegal XhoI site found at 2794
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 2918
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 885
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 2918
Plasmid lacks a suffix.
Illegal XbaI site found at 2933
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal AgeI site found at 581
Illegal AgeI site found at 693
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

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 When the SpeI-PstI DNA fragment from <partinfo>BBa_J61002</partinfo>, containing the mRFP1 gene with a strong RBS and a double terminator, is transferred into <partinfo>pSB1C3</partinfo> (digested SpeI-PstI) the resulting plasmid is BBa_K516999. See <partinfo>pSB1C3</partinfo> for detailed feature information.
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 <!-- Add more about the biology of this part here
 ===Usage and Biology===