Difference between revisions of "Part:BBa K575029:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K575029 short</partinfo>
 
<partinfo>BBa_K575029 short</partinfo>
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<partinfo>BBa_K575029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K575029 SequenceAndFeatures</partinfo>
  
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==Design Notes===
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===Design Notes===
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The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
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put the constitutive promoter after the production of LasR
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===Source===
 
===Source===
  
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from registry parts
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The pieces of this construct are all existing registry parts.
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===References===
 
===References===

Revision as of 01:09, 26 September 2011

LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0034) + LasR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 918
    Illegal NheI site found at 941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1294
    Illegal AgeI site found at 1491
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 827

Design Notes=

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

The pieces of this construct are all existing registry parts.

References