Difference between revisions of "Part:BBa K590060"

Revision as of 01:44, 23 September 2011

LuxD; standalone, E. Coli codon optimized

This part consists of a synthesized version of the Lux C gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It is optimized to have cloning-friendly GC content and also is systemized to fit in Escherichia coli.

Usage and Biology

[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with Lux C and Lux E to engineer alternative routes for fatty aldehyde production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 247
Illegal BsaI site found at 616

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590060 short</partinfo>
-This part consists of a synthesized version of the Lux D gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It was optimized to have cloning-friendly GC content and systemized to fit in ''Escherichia coli''.
+This part consists of a synthesized version of the Lux C gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It is optimized to have cloning-friendly GC content and also is systemized to fit in ''Escherichia coli''.
 ===Usage and Biology===