Difference between revisions of "Part:BBa K590034"
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<partinfo>BBa_K590034 short</partinfo>
 
<partinfo>BBa_K590034 short</partinfo>
  
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This part causes ''Escherichia coli'' to produce branched chain fatty acids.  
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[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM team] has used this part to cause ''Escherichia coli'' to produce branched chain fatty acids.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 00:30, 23 September 2011

FabH2

[http://2011.igem.org/Team:Washington University of Washington 2011 iGEM team] has used this part to cause Escherichia coli to produce branched chain fatty acids.

Usage and Biology

FabH2 is from Bacillus subtilis. The FabH family of proteins initiates fatty acid elongation by converting an Acyl-CoA into an Acyl-ACP, with is extended by 2 carbon units to form longer chain length fatty acids. Normally, FabH proteins use a simple 2-carbon acetyl-CoA to start fatty acid biosynthesis, resulting in linear fatty acids. However, FabH2 can also use Isobutyryl-CoA, Isovaleryl-CoA, and 2-Methylbutyryl-CoA (products from Valine, Leucine, and Isoleucine degredation, resulting in 2-methyl branched fatty acid production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 310
    Illegal AgeI site found at 949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355