Difference between revisions of "Part:BBa K590000"

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<partinfo>BBa_K590000 short</partinfo>
 
<partinfo>BBa_K590000 short</partinfo>
  
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This part includes gene mamH and mamI, which were extracted from the genome of ''Magnetospirillum magneticum'' strain AMB-1  by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].  
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This part includes genes mamH and mamI, which were extracted from the genome of <i>Magnetospirillum magneticum</i> strain AMB-1  by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].  
  
 
===Usage and Biology===
 
===Usage and Biology===
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The gene number of mamH is AMB0961; mam I is AMB0962. Specific function of mamH was not reported. But it was found that mamI associates with the magnetosome chain localization, and the loss of mamI causes no membrane formation.
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The gene number of mamH is AMB0961; mam I is AMB0962. The specific function of MamH has not been reported. MamI associates with the magnetosome for chain localization, and the loss of mamI causes no magnetosome vesicle formation.
  
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The image below shows the band(right) of HI, which is 1600bp.  
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The image below shows the PCR band(right) of HI, which is 1600bp.  
  
 
[[Image:Igem2011 HI gel.png|thumb|center|1kb ladder(left),HI(right)]]
 
[[Image:Igem2011 HI gel.png|thumb|center|1kb ladder(left),HI(right)]]

Revision as of 18:28, 22 September 2011

mamHI, from Magnetospirillum magneticum

This part includes genes mamH and mamI, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

The gene number of mamH is AMB0961; mam I is AMB0962. The specific function of MamH has not been reported. MamI associates with the magnetosome for chain localization, and the loss of mamI causes no magnetosome vesicle formation.

The image below shows the PCR band(right) of HI, which is 1600bp.

1kb ladder(left),HI(right)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 89
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 2589
    Illegal NgoMIV site found at 3920
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal BsaI site found at 1986
    Illegal BsaI site found at 3317
    Illegal SapI site found at 825
    Illegal SapI site found at 2160
    Illegal SapI site found at 3491