Difference between revisions of "Part:BBa K660201:Design"
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<h3>Illegal Restriction Sites</h3> | <h3>Illegal Restriction Sites</h3> | ||
− | We are aware of two illegal restriction sites that exist in our latherin biobrick. Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for | + | We are aware of two illegal restriction sites that exist in our latherin biobrick. Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for use with the Stratagene QuikChange Site-Directed Mutagenesis Kit.<br/><br/> |
<u>1st PstI site:</u><br/> | <u>1st PstI site:</u><br/> |
Revision as of 04:14, 22 September 2011
Latherin (Surfactant Protein) + 6xHIS Tag
Illegal Restriction Sites
We are aware of two illegal restriction sites that exist in our latherin biobrick. Due to time constraints, we were unable to complete site directed mutagenesis. The primers we designed for this are below, for use with the Stratagene QuikChange Site-Directed Mutagenesis Kit.
1st PstI site:
5': GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG
Forward Primer: 5' CAG TTG CAG ACG GGT ATC TTG CAG TTT AAT TTC CGC
Reverse Primer: 5' GCG GAA ATT AAA CTG CAA GAT ACC CGT CTG CAA CTG
MTP= 79.33
2nd PstI site:
5': GCG ATT GAG CTG CAA AGC GGC AAT CCG C
Forward Primer: 5' G CGG ATT GCC GCT TTG CAG CTC AAT CGC
Reverse Primer: 5' GCG ATT GAG CTG CAA AGC GGC AAT CCG C
MTP= 78.71