Difference between revisions of "Part:BBa K606036:Experience"
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[[image:T7 autoloop ctrl-25.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C (trans image) ]] | [[image:T7 autoloop ctrl-25.jpg|450px|thumb|center|E.coli T7 autoloop without terminator at 37°C (trans image) ]] | ||
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[[image:T7 autoloop ctrl-10.jpg|450px|thumb|center|E.coli negative control at 37°C (trans image)]] | [[image:T7 autoloop ctrl-10.jpg|450px|thumb|center|E.coli negative control at 37°C (trans image)]] | ||
[[image:T7 autoloop ctrl-11.jpg|450px|thumb|center|E.coli negative control at 37°C (gfp image)]] | [[image:T7 autoloop ctrl-11.jpg|450px|thumb|center|E.coli negative control at 37°C (gfp image)]] | ||
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+ | These pictures show that the T7 GFP autoloop system is efficient since some cells are glowing with GFP fluorescence. Thus, we can conclude that the T7 polymerase is activated. Finally, we notice there is no difference between the GFP autoloop with and without terminator before the T7 promoter. It could be due to that terminator which is a B.subtilis terminator. Moreover, we know that this E.coli strain has 4 terminators. |
Revision as of 03:58, 22 September 2011
File:File:T7 autoloop ctrl-26.jpg
E.coli T7 autoloop without terminator at 37°C (gfp image)
These pictures show that the T7 GFP autoloop system is efficient since some cells are glowing with GFP fluorescence. Thus, we can conclude that the T7 polymerase is activated. Finally, we notice there is no difference between the GFP autoloop with and without terminator before the T7 promoter. It could be due to that terminator which is a B.subtilis terminator. Moreover, we know that this E.coli strain has 4 terminators.