Difference between revisions of "Part:BBa K606040:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We synthetised it with elongation of two primers that binds each other for 30bp. We shorten the original part.
+
 
 +
This part have been synthetize de novo, by two 80bp primers matching on about 20bp, and completed using phusion polymerase, and then cloned in the pSB1C3 standard plasmid.
  
 
===Source===
 
===Source===

Revision as of 08:48, 26 October 2011

Promoter Hyperspank B.subtilis & E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part have been synthetize de novo, by two 80bp primers matching on about 20bp, and completed using phusion polymerase, and then cloned in the pSB1C3 standard plasmid.

Source

inspired from the part BBa_K143015

References