Difference between revisions of "Part:BBa K541545"

Revision as of 03:56, 22 September 2011

Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)

Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge- shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.

We also tested this part by inserting into E.coli. Thus, E.coli synthesized LALF and stopped its own growth because LALF binds LPS layer on the cell wall. Therefore, we could test whether our protein works or not

B.subtilis with IPTG inducible promoter and LALF in plates with and without IPTG

Control groups in plates with and without IPTG

Sequence and Features