Difference between revisions of "Part:BBa K606040:Experience"

Revision as of 03:56, 22 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K606040

The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter thant the classic one, but is also recognised by E. coli polymerases. We cloned it first behind the amber tRNA that would induce a GFP amber on an other plasmid. Then to characterise it we cloned it behind an RFP, into TURBO cells that are LacIq to allow the promoter to be the less leacky possible. The pictures below are the results of an IPTG induction.

User Reviews

UNIQ51a76dbe235313f6-partinfo-00000000-QINU UNIQ51a76dbe235313f6-partinfo-00000001-QINU

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 ===Applications of BBa_K606040===
-This promoter Hyper Spanck is IPTG inducible.
+The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter thant the classic one, but is also recognised by E. coli polymerases.
-It has been characterised in front of a tRNA amber that would be used in the translation of a GFP amber.
+We cloned it first behind the amber tRNA that would induce a GFP amber on an other plasmid. Then to characterise it we cloned it behind an RFP, into TURBO cells that are LacIq to allow the promoter to be the less leacky possible.
+The pictures below are the results of an IPTG induction.
 ===User Reviews===