Difference between revisions of "Part:BBa K592100"

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'''iGEM11_Uppsala-Sweden'''. Fluorescence on UV table. The images above show ''E coli'' constitutively expressing mTagBFP <partinfo>K592100</partinfo> (blue) and YFP <partinfo>K592101</partinfo> (yellow) illuminated on a UV table.  
 
'''iGEM11_Uppsala-Sweden'''. Fluorescence on UV table. The images above show ''E coli'' constitutively expressing mTagBFP <partinfo>K592100</partinfo> (blue) and YFP <partinfo>K592101</partinfo> (yellow) illuminated on a UV table.  
 
 
 
[[Image:Promoter characterization.png]]
 
 
'''iGEM11_Uppsala-Sweden'''. BFP used as reporter. This part has been successfully used as a reporter gene for promoter characterization using flow cytometry. The excitation peak at 399 nm makes it well suited for use in flow cytometers using a 407 nm violet laser such as the FACSAria II, and the emission peak at 456 works well with a DAPI filter (band pass 450/40). The Y axis measures the promoter activity in RPU, relative promoter units, where the activity of the J23101 promoter is defined as 1 RPU.
 
  
  

Revision as of 22:54, 24 September 2011

Blue Fluorescent Protein (mTagBFP)

This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRFP) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.

Subach et. al. reported the following data:

Excitation peak: 399 nm. Emission peak: 456 nm. Maturation Half-Time (min): 13. Relative brightness to EBFP2: 1.82. Extinction Coefficient (M-1 cm-1): 52000. Quantum Yield: 0.63.

Usage and Biology

This part is useful as a reporter.

A14 UU.JPG A16 UU.jpg

iGEM11_Uppsala-Sweden. Fluorescence on UV table. The images above show E coli constitutively expressing mTagBFP BBa_K592100 (blue) and YFP BBa_K592101 (yellow) illuminated on a UV table.


References

[http://www.ncbi.nlm.nih.gov/pubmed/18940671] Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]