Difference between revisions of "Part:BBa K515007"
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<p><b>This BioBrick has been sequence verified.</p></b>
 
<p><b>This BioBrick has been sequence verified.</p></b>
  
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<h2>Description</h2>
 
<h2>Description</h2>
 
<p>This part consists of coding sequence codon optimised for <i>Escherichia coli</i>. It is part of the composite biobrick <a href="https://parts.igem.org/Part:BBa_K515107">BBa_K515107</a>. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm<sup>[1]</sup>.</p>
 
<p>This part consists of coding sequence codon optimised for <i>Escherichia coli</i>. It is part of the composite biobrick <a href="https://parts.igem.org/Part:BBa_K515107">BBa_K515107</a>. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm<sup>[1]</sup>.</p>
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<h2>Reference</h2>
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<h2>References</h2>
 
[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.
 
[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.
 
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<p><b>This BioBrick has been sequence verified.</p></b>
 

Revision as of 01:48, 22 September 2011

Dendra2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.

Description

This part consists of coding sequence codon optimised for Escherichia coli. It is part of the composite biobrick BBa_K515107. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm[1].

References

[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.