Difference between revisions of "Part:BBa K676011:Design"

(References)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
PCR and then 2 parts ligation
+
Performed PCR to cloned out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.
The GBS in pSC101 plasmid (285 bp) from 4580 to 4864:
+
Entire pSC101 Sequence - 9263 bp
+
 
+
CAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAAT
+
CAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCA
+
GACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTA
+
TGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTAC
+
CCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAA
+
GACAGCGACTCAGGTGCCTGATGGTCGGAG
+
  
 
===Source===
 
===Source===
  
pSC101 plasmid
+
pSC101 Plasmid DNA - 4580 to 4864 bp
  
 
===References===
 
===References===
 
Mark Oram , Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003)A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu , pSC101 and pBR322 Strong gyrase Sites ; the role of DNA sequence in modulating gyrase supercoiling and biological activity ; Molecular Microbiology 50(1).333-347
 
Mark Oram , Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003)A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu , pSC101 and pBR322 Strong gyrase Sites ; the role of DNA sequence in modulating gyrase supercoiling and biological activity ; Molecular Microbiology 50(1).333-347

Revision as of 02:13, 10 October 2011

Gyrase Binding Site from pSC101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Performed PCR to cloned out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.

Source

pSC101 Plasmid DNA - 4580 to 4864 bp

References

Mark Oram , Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003)A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu , pSC101 and pBR322 Strong gyrase Sites ; the role of DNA sequence in modulating gyrase supercoiling and biological activity ; Molecular Microbiology 50(1).333-347