Difference between revisions of "Part:BBa I15010:Experience"

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===Problem with BBa_I15010===
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This part was used by the 2011 Uppsala-Sweden team[http://2011.igem.org/Team:Uppsala-Sweden] as an integral part of the multichromatic light sensing project. Repeated attempts to transform or PCR the DNA from the 2011 iGEM distribution kit failed while all other parts seemed viable. Uppsala-Sweden requested the Jeffrey Tabor's pJT122 plasmid from Chris A. Voigt and amplified the cph8 DNA from there. Tabor's cph8 sequenced contained one internal PstI site, which was subsequently removed. The resultant DNA sequence was identical to that of BBa_I15010. Uppsala-Sweden sequenced this identical DNA part and submitted it with a new BioBrick number: <partinfo>BBa_K592000</partinfo>. Furthermore, Uppsala-Sweden also submitted the part <partinfo>BBa_K592018</partinfo> which is Cph8 with the BBa_B0034 ribosome binding site. This version of Cph8 with RBS has been completely sequenced and submitted to the registry.
 
This part was used by the 2011 Uppsala-Sweden team[http://2011.igem.org/Team:Uppsala-Sweden] as an integral part of the multichromatic light sensing project. Repeated attempts to transform or PCR the DNA from the 2011 iGEM distribution kit failed while all other parts seemed viable. Uppsala-Sweden requested the Jeffrey Tabor's pJT122 plasmid from Chris A. Voigt and amplified the cph8 DNA from there. Tabor's cph8 sequenced contained one internal PstI site, which was subsequently removed. The resultant DNA sequence was identical to that of BBa_I15010. Uppsala-Sweden sequenced this identical DNA part and submitted it with a new BioBrick number: <partinfo>BBa_K592000</partinfo>. Furthermore, Uppsala-Sweden also submitted the part <partinfo>BBa_K592018</partinfo> which is Cph8 with the BBa_B0034 ribosome binding site. This version of Cph8 with RBS has been completely sequenced and submitted to the registry.
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Revision as of 02:14, 22 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I15010

This part was used by the 2009 WashU iGEM team[http://2009.igem.org/Team:Wash_U] as an integral part of their light sensitive regulation of LHII antennae size in Rhodobacter sphaeroides. However, due to its uncertain reliability in the Registry (a PstI cut site still exists in the part), manipulation of DNA containing the part was necessarily limited. The WashU team is attempting to fix and resubmit this part to the registry.

User Reviews

UNIQ7f0184aabe2d194b-partinfo-00000000-QINU

This part was sent to WashU iGEM as an agar slab and it is not of decent quality, as suggested in the Registry quality control information. We are in the process of attempting to resubmit the part.

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ7f0184aabe2d194b-partinfo-00000002-QINU

SunLei

This part was used by the 2011 Uppsala-Sweden team[http://2011.igem.org/Team:Uppsala-Sweden] as an integral part of the multichromatic light sensing project. Repeated attempts to transform or PCR the DNA from the 2011 iGEM distribution kit failed while all other parts seemed viable. Uppsala-Sweden requested the Jeffrey Tabor's pJT122 plasmid from Chris A. Voigt and amplified the cph8 DNA from there. Tabor's cph8 sequenced contained one internal PstI site, which was subsequently removed. The resultant DNA sequence was identical to that of BBa_I15010. Uppsala-Sweden sequenced this identical DNA part and submitted it with a new BioBrick number: BBa_K592000. Furthermore, Uppsala-Sweden also submitted the part BBa_K592018 which is Cph8 with the BBa_B0034 ribosome binding site. This version of Cph8 with RBS has been completely sequenced and submitted to the registry.

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