Difference between revisions of "Part:BBa K091104:Experience"
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We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | ||
However, we see a fragment of 1000bp and one of ±2000bp. | However, we see a fragment of 1000bp and one of ±2000bp. | ||
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Latest revision as of 08:52, 27 September 2011
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[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team] |
Characterization by K.U.Leuven 2011 iGEM Team We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and one of ±2000bp. |
UNIQ485697dadd2c01d1-partinfo-00000002-QINU