Difference between revisions of "Part:BBa K091104:Experience"

Revision as of 22:55, 21 September 2011

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Applications of BBa_K091104

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UNIQ971791dd3b85ecc4-partinfo-00000000-QINU

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[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team]

Characterisation

We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and one of ±2000bp.

UNIQ971791dd3b85ecc4-partinfo-00000002-QINU

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 '''Characterisation'''
-We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI.  Since the pSB1A2  vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp.
+We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI.  Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp.
-However,  we see a fragment of 1000bp and of ±2000bp.
+However, we see a fragment of 1000bp and one of ±2000bp.
 |}
 [[Image:Restriction_1807.jpg]]
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