Difference between revisions of "Part:BBa K091104:Experience"
(→User Reviews) |
(→User Reviews) |
||
| Line 24: | Line 24: | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
'''Characterisation''' | '''Characterisation''' | ||
| + | |||
We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. | ||
However, we see a fragment of 1000bp and of ±2000bp. | However, we see a fragment of 1000bp and of ±2000bp. | ||
Revision as of 22:51, 21 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K091104
User Reviews
UNIQ4d44ab4ca6d8692f-partinfo-00000000-QINU
|
•
SarahV, KULeuven iGEM team 2011 |
Characterisation We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and of ±2000bp. |
UNIQ4d44ab4ca6d8692f-partinfo-00000002-QINU