Difference between revisions of "Part:BBa K676013"
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The higher level of expression can be use in making a more sensitive SPY device in order to detect shear stress level of the cells during fermentation as well as in downstream processing. | The higher level of expression can be use in making a more sensitive SPY device in order to detect shear stress level of the cells during fermentation as well as in downstream processing. | ||
| − | As with the BBa_K676001( Mu GBS ) , we also observed a better uniformity( smaller range of supercoiled topoisomers) in the supercoiled plasmids ( Mu GBS with SPY device) . | + | As with the BBa_K676001( Mu GBS ) , we also observed a slightly higher supercoiling efficiency ( about 10% higher compare to the controls) and better uniformity( smaller range of supercoiled topoisomers) in the supercoiled plasmids ( Mu GBS with SPY device) . |
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Revision as of 22:12, 21 September 2011
Mu/Spy Ligation
A device to detects the level of stress due to a GBS being present on the plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1203