Difference between revisions of "Part:BBa K607027"
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<partinfo>BBa_K607027 short</partinfo> | <partinfo>BBa_K607027 short</partinfo> | ||
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| + | This part is a member of [https://parts.igem.org/LasR_autoinducing_loop_family LasR autoinducing loop family]. | ||
LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose. | LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose. | ||
| + | [[Image:LasR5.png|Caption test]] | ||
| + | [[Image:lciaulegend.png|frameless]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 23:48, 21 September 2011
PlasB_LasR-DAS_antipBad/araC with 0.07RBS
This part is a member of LasR autoinducing loop family.
LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 195
Illegal NheI site found at 1095 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1156
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 644
Illegal AgeI site found at 841
Illegal AgeI site found at 1321 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1338