Difference between revisions of "Part:BBa I757010:Experience"

Revision as of 01:05, 22 September 2011

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Applications of BBa_I757010

Done by iGEM11 Potsdam_Bioware

This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.
When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C, or up to 300 µg/ml ampicillin, when incubated over night at 30 °C.

User Reviews

UNIQ527bd9bc5ad5bbf0-partinfo-00000000-QINU UNIQ527bd9bc5ad5bbf0-partinfo-00000001-QINU The lactamases shows great activity at 37°C and 30°C incubation over night on agar plates. The transformed E.coli cells were able to survive with ampicillin concentrations up to 400 µg/ml at 37°C and at 30°C.

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-The lactamases shows great activity at 37°C and 30°C incubation over night on agar plates. The transformed <i>E.coli</i> cells were able to survive with ampicillin concentrations up to 400 µg/ml at 37°C and up to 300 µg/ml at 30°C.
+The lactamases shows great activity at 37°C and 30°C incubation over night on agar plates. The transformed <i>E.coli</i> cells were able to survive with ampicillin concentrations up to 400 µg/ml at 37°C and at 30°C.