Difference between revisions of "Part:BBa K592101"

Revision as of 22:34, 21 September 2011

Yellow Fluorescent Protein (YFP)

Yellow Fluorescent Protein derived from Aequorea victoria GFP. This sequence is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original gene was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington.

Usage and Biology

This part is useful as a reporter.

The images above show E coli constitutively expressing YFP BBa_K592101 (yellow) and mTagBFP BBa_K592100 (blue) illuminated on a UV table.

References

[http://www.ncbi.nlm.nih.gov/pubmed/12183631] Elowitz, M. B., A. J. Levine, et al. (2002). "Stochastic gene expression in a single cell." Science 297(5584): 1183-6.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
+This part is useful as a reporter.
+[[Image:A15 UU.JPG|400px]]
+[[Image:A16 UU.jpg|400px]]
+The images above show ''E coli'' constitutively expressing YFP <partinfo>K592101</partinfo> (yellow) and mTagBFP <partinfo>K592100</partinfo> (blue) illuminated on a UV table.
 ===References===