Difference between revisions of "Template:ExpressionAdaptor"

Latest revision as of 00:29, 22 September 2011

This part encodes expression adaptor designed to have strength {{{strength}}} relative to B0034 and uniform expression level regardless of attached protein.

Authors

Clonning:

Paweł Urbański
Elżbieta Jankowska
Krzysztof Szczepaniak
Dorota Kaja Sabat

The lab work was supervised by:

Michał Lower
Ania Olchowik

Construct design

The part is in RFC25 standard. It consists of BBa_J23100 promoter followed by BBa_B0034 RBS, 5 nucleotide spacer and short peptide.

Spacer and peptide were designed using in silico evolution to provide uniform expression level regardless of following protein coding sequence.

Summary of design algorithm:

Measurements

Using RBSCalculator program we have determined that this part expression strength is {{{strength}}} relative to BBa_B0034

All our measurement results are summarized in the table below:

Part Name	Description	Expression level in thousands of absolute RBSDesigner units	Expression level as %B0034+YFP
BBa_K528015	Expression Adapters s00_45k	45	64,29%
BBa_K528016	Expression Adapters s01_24k	24	34,29%
BBa_K528017	expression adapters s02_20k	20	28,57%
BBa_K528018	Expression Adapters s06_16k	16	22,86%
BBa_K528019	Expression Adapters s05_12k	12	17,14%
BBa_K528020	expression adapters s03_65k	65	92,86%
BBa_K528021	BBa_K528018+YFP measurement device	16	22,86%
reference	BBa_J23102+B0034+YFP (100% reference construct)	70	100,00%

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/ExpressionAdaptors wiki page].

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 <table class="tableizer-table">
-<tr class="tableizer-firstrow"><th>Part Name</th><th>Description</th><th>Expression level in thousands of absolute RBSDesigner units</th><th>Expression level as %B0034+GFP</th></tr> <tr><td>BBa_K528015</td><td>Expression Adapters s00_45k</td><td>45</td><td>64,29%</td></tr> <tr><td>BBa_K528016</td><td>Expression Adapters s01_24k</td><td>24</td><td>34,29%</td></tr> <tr><td>BBa_K528017</td><td>expression adapters s02_20k</td><td>20</td><td>28,57%</td></tr> <tr><td>BBa_K528018</td><td>Expression Adapters s06_16k</td><td>16</td><td>22,86%</td></tr> <tr><td>BBa_K528019</td><td>Expression Adapters s05_12k</td><td>12</td><td>17,14%</td></tr> <tr><td>BBa_K528020</td><td>expression adapters s03_65k</td><td>65</td><td>92,86%</td></tr> <tr><td>BBa_K528021</td><td>BBa_K528018+YFP measurement device</td><td>16</td><td>22,86%</td></tr> <tr><td>reference</td><td>BBa_J23102+B0034+GFP (100% reference construct)</td><td>70</td><td>100,00%</td></tr></table>
+<tr class="tableizer-firstrow"><th>Part Name</th><th>Description</th><th>Expression level in thousands of absolute RBSDesigner units</th><th>Expression level as %B0034+YFP</th></tr> <tr><td>BBa_K528015</td><td>Expression Adapters s00_45k</td><td>45</td><td>64,29%</td></tr> <tr><td>BBa_K528016</td><td>Expression Adapters s01_24k</td><td>24</td><td>34,29%</td></tr> <tr><td>BBa_K528017</td><td>expression adapters s02_20k</td><td>20</td><td>28,57%</td></tr> <tr><td>BBa_K528018</td><td>Expression Adapters s06_16k</td><td>16</td><td>22,86%</td></tr> <tr><td>BBa_K528019</td><td>Expression Adapters s05_12k</td><td>12</td><td>17,14%</td></tr> <tr><td>BBa_K528020</td><td>expression adapters s03_65k</td><td>65</td><td>92,86%</td></tr> <tr><td>BBa_K528021</td><td>BBa_K528018+YFP measurement device</td><td>16</td><td>22,86%</td></tr> <tr><td>reference</td><td>BBa_J23102+B0034+YFP (100% reference construct)</td><td>70</td><td>100,00%</td></tr></table>
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