Difference between revisions of "Part:BBa K525710:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This is the complete sequence of NAD | + | This is the complete sequence of NAD<sup>+<sup> -dependent DNA ligase with PT7, RBS and His-tag |
===Source=== | ===Source=== | ||
Revision as of 20:17, 21 September 2011
DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1449
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1663
Design Notes
This is the complete sequence of NAD+ -dependent DNA ligase with PT7, RBS and His-tag
Source
PCR on the Escherichia coli TOP 10 DNA cloned into BioBrick Vectors
Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
fwd:
rev:
===References===