Difference between revisions of "Part:BBa K615000"

Revision as of 22:26, 24 September 2011

E. coli strain for His3-URA3 one-hybrid selection system

This strain is designed to test zinc finger binding using a one-hybrid metabolic selection system. The endogenous genes HisB and PyrF have been knocked out and replaced with the yeast versions His3 and URA3 under the control of a Zif268 binding site. The gene coding for the omega subunit of RNA polymerase (rpoZ) was knocked out by replacing it with a Zeocin cassette, and the strain is designed to be used in conjunction with BBa_K615001 (zinc finger-omega subunit expression construct).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 845
Illegal BglII site found at 965
Illegal BglII site found at 1478
Illegal BglII site found at 1538
Illegal BamHI site found at 1768
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal PstI site found at 1762
Illegal NgoMIV site found at 181
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2458
Illegal SapI site found at 121
Illegal SapI site found at 331
Illegal SapI.rc site found at 2305

Functional Parameters

biology	grow only at 30⁰C
genotype	l-prophage::bioA/bioB cmR::mutS ΔHisBΔPyrFΔrpoZ + His3+URA3
resistance	Ampicillin, Chloramphenicol, Kanamycin, Zeocin

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 __NOTOC__
 <partinfo>BBa_K615000 short</partinfo>
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-<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K615000 parameters</partinfo>
-<!-- -->
+[[File: HARVstrainsummary.png]]