Difference between revisions of "Part:BBa K638202:Experience"

Revision as of 19:26, 21 September 2011

Applications of BBa_K638202

The purified his-tagged Reflectin A1 has optical properties, best viewed when made into a thin film. The original method for making reflectin thin films was developed by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.], and then [http://2011.igem.org/Team:Cambridge/Experiments/Thin_Films repeated and modified by the Cambridge iGEM Team 2011].

First the protein must be dissolved in an ionic solvent such as [http://en.wikipedia.org/wiki/Hexafluoro-2-propanol HFIP] (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work. A razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a [http://2011.igem.org/Team:Cambridge#/Protocols/Flow_coating flow coater] or a [http://2011.igem.org/Team:Cambridge#/Protocols/Spin_Coating spin coater device].

Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.]]]
Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.] ]]

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UNIQe9b337bf110a2612-partinfo-00000001-QINU UNIQe9b337bf110a2612-partinfo-00000002-QINU

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 ===Applications of BBa_K638202===
-The purified his-tagged Reflectin A1 has optical properties, best viewed when made into a thin film.
+The purified his-tagged Reflectin A1 has optical properties, best viewed when made into a thin film. The original method for making reflectin thin films was developed by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.], and then [http://2011.igem.org/Team:Cambridge/Experiments/Thin_Films repeated and modified by the Cambridge iGEM Team 2011].
-First the protein must be dissolved in an ionic solvent such as HFIP (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work, a razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a flow coater or a spin coater device.
-The original method for making reflectin thin films was developed by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]
+First the protein must be dissolved in an ionic solvent such as [http://en.wikipedia.org/wiki/Hexafluoro-2-propanol HFIP] (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work. A razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a [http://2011.igem.org/Team:Cambridge#/Protocols/Flow_coating flow coater] or a [http://2011.igem.org/Team:Cambridge#/Protocols/Spin_Coating spin coater device].
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