Difference between revisions of "Part:BBa K554000"

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===References===
 
===References===
  
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#Hidalgo E, Leautaud V, Demple B (1998) The redox-regulated SoxR protein acts from a single DNA site as a repressor and an allosteric activator. The EMBO Journal 17(9)2629–2636
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#Hidalgo E, Leautaud V, Demple B (1998) The redox-regulated SoxR protein acts from a single DNA site as a repressor and an allosteric activator. The EMBO Journal 17(9)2629–2636 [http://www.ncbi.nlm.nih.gov/pubmed/?term=9564045%20 Link to Pubmed]

Revision as of 19:10, 21 September 2011

SoxS promoter

SoxS is a promoter regulated by the SoxR transcription factor, which is activated by NO. E. coli can naturally respond to redox signals through a superoxide stress system composed by SoxR gene, SoxS promoter and the genes associated with SoxS promoter. Thus, in the presence of NO, SoxR activates transcription of the gene regulated by SoxS promoter (Hidalgo et al, 1998).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This promoter is used by [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project/Device2 UNICAMP-EMSE Brazil team] in the NO sensor device ("Device 2", which senses NO levels and responds by producing and secreting IL-10. This part is shown in the red box in the following schema:

UNICAMP EMSE NO device schema.jpg

References

  1. Hidalgo E, Leautaud V, Demple B (1998) The redox-regulated SoxR protein acts from a single DNA site as a repressor and an allosteric activator. The EMBO Journal 17(9)2629–2636 [http://www.ncbi.nlm.nih.gov/pubmed/?term=9564045%20 Link to Pubmed]