Difference between revisions of "Part:BBa K546002"

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<partinfo>BBa_K546002 short</partinfo>
 
<partinfo>BBa_K546002 short</partinfo>
  
This device consists of two protein generators.
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This device consists of two protein generators:
 
The first generator  ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein.  
 
The first generator  ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein.  
 +
 
The second generator  ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored.
 
The second generator  ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored.
It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR.
+
 
 +
It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:58, 21 September 2011

Lux pL controlled luxR with lux pR controlled GFP(lva tag).

This device consists of two protein generators: The first generator ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein.

The second generator ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored.

It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828