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Line 2: |
| | <partinfo>BBa_K607001 short</partinfo> | | <partinfo>BBa_K607001 short</partinfo> |
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| − | cI with a fast degradation tag (LVA) under the control of the pBAD/araC promoter. This part is a cI-LVA generator when it is induced by arabinose. | + | cI with a fast degradation tag (LVA) under the control of the PBad promoter |
| − | The pBAD promotor is a promotor which belongs to the arabinose operon and its regulatory gene, araC.(1) This regulatory gene is both a positive and negative regulator (2), so in the presence of arabinose, the transcription from the pBAD will be turned on and in its absence, the transcription of the gene cloned downstream of the pBAD promotor occurs at very low levels. (1)
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| − | <br>
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| − | The cI gene codes for the bacteriophage λ repressor(3). This bacteriophage λ-repressor from phage lambda also contains a ssrA tag. This ssrA tag is located at the C-terminal end of the incomplete protein product of a stalled ribosome. This tag is a target for proteolytic enzymes and causes degradation of the protein.(4)
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| − | <br>
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| − | <br> References:
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| − | <br>(1) Luz-Maria Guzman, Dominique Belin, Michael J. Carson and Jon Beckwith. Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBAD Promoter. Journal of bacteriology, July 1995.
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| − | <br>(2) Wanzhi Huang, Matthew McKevitt and Timothy Palzkill. Use of the arabinose pbad promoter for tightly regulated display of proteins on bacteriophage. Department of Molecular Virology & Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
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| − | <br>(3)Robert T. Sauer* and Robert Anderegg. Primary Structure of the λ Repressor. Biochemistry, 1978.
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| − | <br>(4) Jeff Hasty, David McMillen, Farren Isaacs and James J. Collins. Computational studies of gene regulatory networks: in numero molecular biology. Nature 268
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