Difference between revisions of "Part:BBa K613022"
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<partinfo>BBa_K613022 short</partinfo> | <partinfo>BBa_K613022 short</partinfo> | ||
− | This is a TetR mutant with the | + | This is a TetR mutant with the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant. |
+ | |||
+ | '''Characterization''' | ||
+ | The mutant was characterized in vitro by | ||
+ | |||
+ | and in vivo | ||
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Revision as of 18:24, 21 September 2011
TetR E37A P39K mutant
This is a TetR mutant with the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant.
Characterization The mutant was characterized in vitro by
and in vivo
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Designed sequence (2 mutations in yellow):
MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQKTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFD
HQGAEPAFLFGLELIICGLEKQLKCESGS*