Difference between revisions of "Part:BBa K627008"

Revision as of 15:49, 21 September 2011

Fusion part of arabinose-inducible induction system and the TEV protease

This BioBrick is a 2 parts fusion of pBAD arabinose-inducible induction system and the TEV protease. TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a endopeptidase (NIa) encoded by the tobacco etch virus (TEV). TEV protease is a useful reagent for cleaving fusion proteins. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. In TEV protease the serine nucleophile of the conventional Ser-Asp-His triad is a cysteine instead. This probably explains why TEV protease is resistant to many commonly used protease inhibitors. At 37°C, the TEV protease forms inculsion body, which leads to an inactive form. Incubated at 30 °C, the protease is expressed as soluble type and is highly active. The induction system was amplified via PCR und fused via NgoMIV with the protease.

Sequence and Features