Difference between revisions of "Part:BBa K549003"
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The promoter is also a little bit sensitive for Co 2+. | The promoter is also a little bit sensitive for Co 2+. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This is how the BioBrick is working: | ||
| + | [[Image:Bild-rcnaluxAB.png]] | ||
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Revision as of 15:37, 21 September 2011
Nickel(II) dependent promotor with luxAB as reporter
The biobrick contains the RcnA promotor from E. coli fused with the luxAB reporter gene. E coli strains own the RcnR-regulator gene which direct regulates the transcription of the RcnA nickel cobalt efflux protein from which the promotor is amplified. In presence of nickel the Luciferase is expressed.
The promoter is also a little bit sensitive for Co 2+.
Usage and Biology
This is how the BioBrick is working:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 751
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1270