Difference between revisions of "Part:BBa K549001"
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This is how it works: | This is how it works: | ||
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The detector is also sensing Co 2+, but not very sensitivly. | The detector is also sensing Co 2+, but not very sensitivly. | ||
Revision as of 15:33, 21 September 2011
Ni(II) dependent rcnA-promoter and GFP
E. coli has an RcnR-regulator that switches on the RcnA-Promotor in the presence of Ni(II). After induction GFP will be expressed.
E. coli has to be used as reporter organism since RcnR is not coded on the plasmid.
Usage and Biology
This BioBrick can be used to detect Ni(II)-Ions in water.
This is how it works:
The detector is also sensing Co 2+, but not very sensitivly.
Sources:
- [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05369.x/pdf Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function] Iwig JS, Rowe JL, Chivers PT
- [http://www.ncbi.nlm.nih.gov/pubmed?term=coordinating%20intracellular%20chivers Coordinating intracellular nickel-metal-site structure-function relationships and the NikR and RcnR repressors] Iwig JS, Chivers PT
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 863