Difference between revisions of "Part:BBa I742111:Experience"

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===User Reviews===
 
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<I>iGEM Groningen 2009</I>
 
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The capacity of fMT to enhance the arsenite accumulation in ''E. coli'' was tested using an arsenite uptake [http://2009.igem.org/Team:Groningen/Protocols assay]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in ''E. coli'' carrying fMT (induced by lactose).
 
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Revision as of 15:32, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I742111

A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this page.

User Reviews

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iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562018). This will allow better monitoring of production of the protein. We did not get the chance to test arsenic resistance in a host cell producing this part.

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