Difference between revisions of "Part:BBa I746907:Experience"

(→‎our GFP Assay)
(→‎TU_Munich Team 2011)
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We tested the part and it worked very well.
 
We tested the part and it worked very well.
 
[[Image:2011-09-19 GFP Assay Diagramm prozentual.jpg]]
 
[[Image:2011-09-19 GFP Assay Diagramm prozentual.jpg]]
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==our GFP Assay==
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I746907  (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells
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change of medium to M63 (LB shows slight fluorescence itself)
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'''Problem''': measurement of Absorbance only every 100 minutes led to spikes in the diagram
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====results====
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no concentration dependency of GFP induction as IPTG concentration was in saturation range
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for this diagram:
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* calculated: Fluorescence/Absorbance
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* mean of triplicate measurement
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* adjusted to 100 %
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* corrected for outliers
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* shortened to 700 min
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<!-- DON'T DELETE --><partinfo>BBa_I746907 StartReviews</partinfo>
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<!-- Template for a user review
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{|width='80%' style='border:1px solid gray'
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|-
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|width='10%'|
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<partinfo>BBa_I746907 AddReview number</partinfo>
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<I>TU_Munich team 2011</I>
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|width='60%' valign='top'|
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enter your review here.
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|};
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<!-- End of the user review template -->
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<!-- DON'T DELETE --><partinfo>BBa_I746907 EndReviews</partinfo>

Revision as of 14:26, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I746907

User Reviews

TU_Munich Team 2011

We tested the part and it worked very well. 2011-09-19 GFP Assay Diagramm prozentual.jpg


our GFP Assay

I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells

change of medium to M63 (LB shows slight fluorescence itself)

Problem: measurement of Absorbance only every 100 minutes led to spikes in the diagram

results

no concentration dependency of GFP induction as IPTG concentration was in saturation range

for this diagram:

  • calculated: Fluorescence/Absorbance
  • mean of triplicate measurement
  • adjusted to 100 %
  • corrected for outliers
  • shortened to 700 min


UNIQe755f847bee8e32c-partinfo-00000000-QINU UNIQe755f847bee8e32c-partinfo-00000001-QINU