Difference between revisions of "Part:BBa K676001:Experience"
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'''The level of supercoiling achieved''' | '''The level of supercoiling achieved''' | ||
| − | The plasmids with Mu GBS travelled further compared to the control . This indicated that the Mu GBS enables a higher supercoiled topoisomers to be formed . Overall ,the level of negative supercoiling | + | The plasmids with Mu GBS travelled further compared to the control . This indicated that the Mu GBS enables a higher supercoiled topoisomers to be formed .There was also one distinct band which migrate the furthest from the wells and bands in the Mu GBS lane , this indicate that the introduction of Mu GBS can result in the formation of a highly supercoiled plasmids . Overall ,the level of negative supercoiling achieved with the Mu SGS was: |
Calculations: By comparing the length of travel of the bands from the well in each samples | Calculations: By comparing the length of travel of the bands from the well in each samples | ||
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Control= 9.6cm | Control= 9.6cm | ||
| − | Mu GBS = | + | Mu GBS = 13.7cm |
| − | Difference: ( | + | Difference: (4.2/9.6)(100) = 42.7% |
'''Quality of supercoiling:''' | '''Quality of supercoiling:''' | ||
Revision as of 14:05, 21 September 2011
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Applications of BBa_K676001
According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the Mu phage GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.
The level of supercoiling achieved The plasmids with Mu GBS travelled further compared to the control . This indicated that the Mu GBS enables a higher supercoiled topoisomers to be formed .There was also one distinct band which migrate the furthest from the wells and bands in the Mu GBS lane , this indicate that the introduction of Mu GBS can result in the formation of a highly supercoiled plasmids . Overall ,the level of negative supercoiling achieved with the Mu SGS was:
Calculations: By comparing the length of travel of the bands from the well in each samples
Control= 9.6cm
Mu GBS = 13.7cm
Difference: (4.2/9.6)(100) = 42.7%
Quality of supercoiling: Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled Mu GBS plasmids increased by close to 30%( if the single distinct band was not included) compared to the control. Calculations: By comparing the range of topoisomers travel distances ( between the "slowest" and "fastest")
Control=4.9cm "smear"
Mu GBS =3.4cm "smear"
Difference: (1.5/4.9)(100%) = 30.6%
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