Difference between revisions of "Part:BBa K568000"

Revision as of 17:27, 21 September 2011

Red light sensor

Single red light inducible construct without downstream genes. This part can be cloned upstream of any desired gene product.

Usage and Biology

Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene. This part needs an EnvZ deficient strain such as CP919 to function properly, as EnvZ pathway is supposedly also responsible for sensing of aspartate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 644
Illegal XhoI site found at 1786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 971
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

n/a	Red light sensor

Induction with red light at 705 nm.

Since OmpR is expressed phosphorylated and therefore gene expression is induced in the dark, red light signalling can be shut down with 650 nm.[1]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene. This part needs an envZ deficient strain such as CP919 to function properly, as EnvZ pathway is supposedly also responsible for sensing of aspartate.
+Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene. This part needs an EnvZ deficient strain such as CP919 to function properly, as EnvZ pathway is supposedly also responsible for sensing of aspartate.
 <span class='h3bb'>Sequence and Features</span>