Difference between revisions of "Part:BBa K676001:Experience"
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According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the Mu phage GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel. | According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the Mu phage GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel. | ||
| − | Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled | + | '''The level of supercoiling achieved''' |
| + | The plasmids with Mu GBS travelled further compared to the control . This indicated that the Mu GBS enables a higher supercoiled topoisomers to be formed . There was a distinct highly negatively supercoiled plasmids band with a "smeared" thicker bands above it. Overall ,the level of negative supercoiling ahcieved with the Mu SGS was | ||
| + | |||
| + | Calculations: By comparing the length of travel of the bands from the well in each samples | ||
| + | Control= 9.6cm | ||
| + | Mu GBS = 10.7cm | ||
| + | Difference: | ||
| + | |||
| + | '''Quality of supercoiling:''' | ||
| + | Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled Mu GBS plasmids increased by close to 30%( if the single distinct band was not included) compared to the control. | ||
| + | Calculations: By comparing the range of topoisomers travel distances ( between the "slowest" and "fastest") | ||
| + | Control=4.9cm "smear" | ||
| + | Mu GBS =3.4cm "smear" | ||
| + | Difference: (1.5/4.9)(100%) = 30.6% | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:55, 21 September 2011
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Applications of BBa_K676001
According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the Mu phage GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.
The level of supercoiling achieved The plasmids with Mu GBS travelled further compared to the control . This indicated that the Mu GBS enables a higher supercoiled topoisomers to be formed . There was a distinct highly negatively supercoiled plasmids band with a "smeared" thicker bands above it. Overall ,the level of negative supercoiling ahcieved with the Mu SGS was
Calculations: By comparing the length of travel of the bands from the well in each samples Control= 9.6cm Mu GBS = 10.7cm Difference:
Quality of supercoiling: Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled Mu GBS plasmids increased by close to 30%( if the single distinct band was not included) compared to the control. Calculations: By comparing the range of topoisomers travel distances ( between the "slowest" and "fastest") Control=4.9cm "smear" Mu GBS =3.4cm "smear" Difference: (1.5/4.9)(100%) = 30.6%
User Reviews
UNIQ51aca2ab2d1b6f5f-partinfo-00000000-QINU UNIQ51aca2ab2d1b6f5f-partinfo-00000001-QINU