Difference between revisions of "Part:BBa K627013:Design"

 
(Design Notes)
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K627013 short</partinfo>
 
<partinfo>BBa_K627013 short</partinfo>
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===Design Notes===
 
===Design Notes===
 +
This biobrick was built by PCR using the following PCR primers:<br>
  
+
*p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
 
+
*o_bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC
 
+
  
 
===Source===
 
===Source===

Revision as of 16:44, 21 September 2011

Fusion of ssTorA, cleavage site of 14_3C protease and b-lactamase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 143
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 796


Design Notes

This biobrick was built by PCR using the following PCR primers:

  • p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
  • o_bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC

Source

References